Concept:The blank needs a preposition showing contrast or concession.
Explanation:The sentence means Helen had inabilities, yet she learnt many things.
This is a contrast, not a cause.
'In spite of' means despite, so it correctly shows contrast.
'Because of' and 'Due to' show cause, which is incorrect here.
'Although' is a conjunction and cannot be used before a noun phrase like 'her inabilities'.
So, 'In spite of' is the correct preposition.
Answer:In spite of